Sample Preparation for Flow Cytometry to Measure pHluorin2 Fluorescence in Living <i>Trypanosoma brucei</i>

doi:10.3791/201368

JoVE Journal > Immunology and Infection

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Immunologie et infection

살아있는 트리파노소마 브루세이(Trypanosoma brucei)에서 pHluorin2 형광을 측정하기 위한 유세포 분석을 위한 시료 전처리

Published: January 19, 2024

doi:

10.3791/201368

Daniel Call*¹, Sabrina S. Pizarro*^2,3, Erica Tovey, Emily Knight³, Carrie Baumgardner⁴, Kenneth A. Christensen, James C. Morris

¹Department Chemistry and Biochemistry,Brigham Young University, ²Eukaryotic Pathogens Innovation Center,Clemson University, ³Department of Genetics and Biochemistry,Clemson University, ⁴Department of Physics and Astronomy,Clemson University

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Sample Preparation for Flow Cytometry to Measure pHluorin2 Fluorescence in Living Trypanosoma brucei. J. Vis. Exp. (Pending Publication), e201368, doi:10.3791/201368 (2024).

살아있는 트리파노소마 브루세이(Trypanosoma brucei)에서 pHluorin2 형광을 측정하기 위한 유세포 분석을 위한 시료 전처리

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살아있는 트리파노소마 브루세이(Trypanosoma brucei)에서 pHluorin2 형광을 측정하기 위한 유세포 분석을 위한 시료 전처리

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