Analysis of Hsp70 Chaperone Activity Using SDS PAGE and Western Blot Analyses of Transformed E. coli Protein

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Biochimie

Análise da atividade de chaperona Hsp70 usando SDS PAGE e Western Blot da proteína transformada de E. coli

Published: March 08, 2024

doi:

10.3791/201543

Hloniphani Rachel Ncube, Unarine Dali, Tendamudzimu Harmfree Dongola, Addmore Shonhai

¹Department of Biochemistry & Microbiology,University of Venda

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Keywords: Hsp70 Chaperone SDS-PAGE Western Blot Transformed E. Coli Protein Analysis Laemmli SDS Loading Buffer Electrophoresis Coomassie Staining Destaining Protein Transfer Nitrocellulose Membrane Antibody Incubation TBS-Tween Buffer Secondary Antibody Chemiluminescence Detection Gel Imaging

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Analysis of Hsp70 Chaperone Activity Using SDS PAGE and Western Blot Analyses of Transformed E. coli Protein. J. Vis. Exp. (Pending Publication), e201543, doi:10.3791/201543 (2024).

Análise da atividade de chaperona Hsp70 usando SDS PAGE e Western Blot da proteína transformada de E. coli

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