Mating and Egg Staging: A Method to Generate Embryos and Sort Them by Developmental Stage
Published: April 30, 2023
Abstract
Source: Hostelley T.L., et al. Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish. J. Vis. Exp. (2017)
The article describes a protocol to generate zebrafish embryos through natural mating and sorting their developmental stages. The protocol is used further for analysis of pancreatic β-cells by using transgenic fishes.
Protocol
1. Generating embryos through natural mating Culture embryos to 3 months of age, reproductive maturity. Segregate adult male and female fish from the desired strain into divided mating tanks on the evening before embryo collection, and add 2 males and 3 females to each tank. NOTE: Use of the transgenic insulin2a:mCherry fluorescent reporter strain allowed the analysis of pancreatic β-cells. Transfer fish to the …
Materials
| ins2a:mCherry | ZIRC | ZL1483 | |
| Mating tanks 1.0L Crossing Tank Set | Aquaneering | ZHCT100 | |
| Dissecting Microscope | Zeiss |
Citer Cet Article
Mating and Egg Staging: A Method to Generate Embryos and Sort Them by Developmental Stage. J. Vis. Exp. (Pending Publication), e20183, doi: (2023).