This video describes an electroporation-based method for encapsulating siRNA into exosomes. The siRNA-loaded exosomes can then be used for therapeutic applications.
Protocol
1. siRNA Encapsulation into Exosomes by Electroporation Pre-chill the electroporation cuvette (see Table of Materials) on ice for 30 min before electroporation. Mix 7.0 µg of exosomes (32 µL from 7 x 1012 p/mL stock in PBS) with 0.33 µg of siRNA (12 µL from 2 µM stock in RNase-free water) in the microcentrifuge tube. Make up the volume to 150 µL with citric acid buffer (see Table of Materials). The exosome to…
siRNA Encapsulation into Exosomes Using Electroporation: An Electric Pulse Based Technique to Load siRNA into Exosomes. J. Vis. Exp. (Pending Publication), e20566, doi: (2023).