Sinoatrial Node Isolation from Mouse Model: A Procedure to Harvest SA Node from Murine Heart
Published: April 30, 2023
Abstract
Source: Kumar, P et al., Microelectrode Array Recording of Sinoatrial Node Firing Rate to Identify Intrinsic Cardiac Pacemaking Defects in Mice. J. Vis. Exp. (2021)
This video demonstrates the procedure to isolate sinoatrial node or SAN from mouse heart. The SAN houses the sinoatrial cells that initiates heartbeat by generating spontaneous action potentials.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Dissecting the sinoatrial node (SAN) Prepare heparinized Complete Tyrode's solution for SAN dissection. Add 400 µL of heparin (1,000 USP/mL) to 40 mL of Complete Tyrode's solution and warm in a 37 °C water bath. Inject the mouse intraperitoneally with 200-300 μL of heparin (1000…
Representative Results
Figure 1: Removal of the heart. (A) Transverse incision in the skin just beneath the bottom of the rib cage from about the left costal arch to the right costal arch. (B) Peritoneal incision. (C,D) Incision of the diaphragm along the thorax to expose the thoracic cavity. (E) Removal of the heart following …
Disclosures
The authors have nothing to disclose.
Materials
| 22 gauge syringe needle | Fisher Scientific | 14-826-5A | Used for dissection |
| 23 gauge syringe needle | Fisher Scientific | 14-826-6C | Used for dissection |
| 60mm Petri Dishes | Genesee Scientific | 32-105G | |
| Castroviejo Scissors, 4" | Fine Science Tools | 15024-10 | |
| Dissection Microscope | Jenco | ||
| Dissecting Pins | Fine Science Tools | 26002-20 | |
| Dumont #2 Laminectomy Forceps | Fine Science Tools | 11223-20 | |
| Dumont #55 Forceps | Fine Science Tools | 11295-51 | |
| Extra Fine Graefe Forceps | Fine Science Tools | 11152-10 | |
| Hemostat | Fine Science Tools | 13013-14 | |
| Heparin | Aurobindo Pharma Limited IDA, Pashamylaram | NDC 63739-953-25 | |
| Sylgruard Elastomer Kit | Dow Corning | 184 SIL ELAST KIT 0.5KG | |
| Surgical Scissors | Fine Science Tools | 14074-09 | |
| Transfer Pipets (3mL graduated) | Samco Scientific | 225 | |
| Isoflurane | Patterson Veterinary | 07-893-1389 |
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Cite This Article
Sinoatrial Node Isolation from Mouse Model: A Procedure to Harvest SA Node from Murine Heart. J. Vis. Exp. (Pending Publication), e20791, doi: (2023).