This video demonstrates the establishment of lung infection in an immunocompetent rodent model via intubation and installation of bacterial suspensions into lung lobes. This model enables antibacterial efficacy studies in immunocompetent animals.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Anesthetize, Intubate, and Inoculate Animals NOTE: Specific pathogen-free (SPF) immunocompetent male Sprague-Dawley rats weighing 100 – 120 g are recommended. Provide all animals with food and water ad libitum and house them socially on wood chips or other absorbent bedding with s…
Representative Results
Figure 1: Nonsurgical Intratracheal Intubation. Tools are shown for intubating the rat (A) and the mouse (B). Once anesthetized, the animal should be positioned as shown in C, with the head to the right and tail to the left if the individual performing the technique is right-handed. Place the left forefinger gently on the throat …
Divulgations
The authors have nothing to disclose.
Materials
Guide Tool
Intek Services Ltd
GT01
1 mL TB syringe slip tip
Becton Dickinson and Company
309659
PrecisionGlide Needle 25 G x 5/8
Becton Dickinson and Company
305122
Intramedic polyethylene tubing
Clay Adams brand – Becton Dickinson and Company
427401
Plastic cannula for mouse
Animal feeding needle – straight 20 x 3" 2-1/4
Popper and Sons, Inc
7903
Used to create metal cannula for mice
75 TN 5.0 µL syringe 26s/2"/2
Hamilton
87930
HPLC glass injection syringe
Difco Nutrient Agar
Becton Dickinson and Company
213000
Pyrex tube, culture 25×150 screwcap with rubber line