A Platelet-Rich Plasma Injection into the Sectioned Achilles Tendon of a Rat
Published: May 31, 2024
Abstract
Source: Greimers, L., et al. Effects of Allogeneic Platelet-Rich Plasma (PRP) on the Healing Process of Sectioned Achilles Tendons of Rats: A Methodological Description. J. Vis. Exp. (2018).
This video demonstrates a technique for injecting platelet-rich plasma (PRP) into the sectioned Achilles tendon of rats. A small segment is excised from the Achilles tendon of a recipient rat, the site is sutured, and the rat is allowed to recover. Concurrently, whole blood is collected from a donor rat and centrifuged to obtain PRP, which is then activated and injected into the sutured site of the recipient rat to induce tendon repair.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Animal Preparation Use 132 2-month-old male Sprague-Dawley rats weighing 320-450 g (120 rats for experimentation and 12 rats for blood sampling, Figure 1). Fifteen rats for each group was enough for a power of 0.8 (80% chance of finding statistical significance if the specified effect exists). <li…
Representative Results
Figure 1: Experimental design of the study. Figure 2: Surgical Procedure. (a) The tendon complex after removal of the surrounding fascia. (b) Removal of the planta…
Divulgations
The authors have nothing to disclose.
Materials
| Xylazine (Xyl-M) | VMD | none | anesthetic |
| Ketamin (Jétamine 1000 CEVA) | CEVA Santé Animale | none | anesthetic |
| Buprenorphin (Vetergésic Multidosis) | ALSTOE | none | Painkiller |
| iso-Betadine | MEDA-Pharma | none | Desinfectant |
| Resorbable yarn Vicryl 6/0 | Johnson & Johnson | ||
| Nembutal | CEVA Santé Animale | none | Anesthetic |
| Paraformaldehyde | Sigma-Aldrich | P6148 | Preserves structure of the tissue |
| Isopropanol 100% | VWR | 2,09,22,364 | |
| Ethanol 95% | VWR | 2,08,23,362 | |
| Xylene | VWR | 28973.363 | |
| Paraffin | VWR | LEIC3950.1006 | |
| Hematoxylin | Millipore | 1.15938.0025 | Colorant |
| Eosin | Millipore | 1.15935.0100 | Colorant |
| Eukitt | Sigma-Aldrich | 3989 | Mounting Medium |
| CaCl2 |
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Citer Cet Article
A Platelet-Rich Plasma Injection into the Sectioned Achilles Tendon of a Rat. J. Vis. Exp. (Pending Publication), e22265, doi: (2024).