In insects, the oenocytes produce cuticular hydrocarbon compounds. These compounds protect against desiccation and facilitate chemical communication. Here we demonstrate a dissection technique used to isolate the oenocytes from adult Drosophila melanogaster, and illustrate how this preparation can be utilized to study genes involved in hydrocarbon synthesis.
In this video article we present a detailed dissection protocol for the preparation of the oenocytes from adult D. melanogaster in a manner suitable for molecular analysis. An abbreviated text-based account of the dissection method has been described elsewhere2, and the resulting oenocyte preparation has been demonstrated to be appropriate for the extraction of both RNA and protein2. Using this preparation it may also be possible to develop methods for the culturing of explanted oenocytes….
The authors have nothing to disclose.
We would like to thank Amsale Belay for her assistance in filming of this video article. This work was funded by CIHR, NSERC and CRC grants to JDL.
Material Name | Type | Company | Catalogue Number | Comment |
---|---|---|---|---|
Sylgard | Dow Corning | |||
Tungsten Wire | Ted Pella, Inc. | 27-11 | 0.005”/20’ | |
Forceps | Dumont | 11252-30 | #5 | |
Shields and Sang M3 Insect Medium | Sigma | S3652 | ||
RNeasy Micro Kit | Qiagen | 74004 | ||
qScript cDNA Synthesis Kit | Quanta Biosciences | 95047-100 | ||
Monoject Hypodermic Needle | Harvard Apparatus | 722289 | 20G1 with aluminum hub |