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In utero Intraventrikulär direktinsprutning och elektroporation av E15 musembryon
JoVE Journal
Biology
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JoVE Journal
Biology
In Utero Intraventricular Injection and Electroporation of E15 Mouse Embryos
Please note that all translations are automatically generated.
Click here for the English version.
In utero Intraventrikulär direktinsprutning och elektroporation av E15 musembryon
DOI:
10.3791/239-v
•
26:17 min
•
July 19, 2007
•
William Walantus
,
David Castaneda
,
Laura Elias
,
Arnold Kriegstein
1
Institute for Regeneration Medicine
,
University of California, San Francisco – UCSF
Chapters
00:00
Title
00:17
Introduction
01:17
Tools and Reagents
02:33
Anesthesia Procedure
03:13
Preparation of Microinjection Pipets
07:18
Prepping Rat for Surgery
09:59
Removing Uterus and Exposing Embryos
12:11
In utero Injection and Electroporation
16:49
Placing Embryos Back Inside Dam
23:46
Conclusion
26:01
End Credits
Tags
In-utero
Intraventricular Injection
Electroporation
E15 Mouse Embryos
Neocortex Manipulation
Progenitors
Lateral Ventricle
Corticogenesis
Gene Over-expression
Gene Knockdown
Cellular Proliferation
Migration
Differentiation
Knockout Strategies
Temporal Window
Video Protocol
Surgery Preparation
Uterine Horns Exposure
DNA Injection
Progenitor Electroporation
Animal Care Post-surgery
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