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Chapters
- 00:05Title
- 02:46Isolation of Primary Cultured Human Normal Mammary Fibroblasts
- 04:43Generation of GFP-labeled, Puromycin-Resistant and Immortalized Human Normal Mammary Fibroblasts
- 07:35Co-injection of Human Mammary Fibroblasts with Breast Carcinoma Cells into an Immunodeficient Mouse
- 08:58Extraction of CAFs or their Corresponding Control Fibroblasts from the Tumor or Fibroblast Xenografts, Respectively
- 13:31Representative Images of Experimentally Generated CAFs
- 15:18Conclusion
Karsinom-assosiert fibroblaster (kafeer) rik på myofibroblasts stede i tumor stroma, spille en viktig rolle i å drive tumor progresjon. Vi utviklet en coimplantation svulst xengraft modell for eksperimentelt generere kafeer fra menneskelige mammary fibroblaster. Protokollen beskriver hvordan å etablere CAF myofibroblasts at få en evne til å fremme tumourigenesis.
Tags
Carcinoma-associated FibroblastsCAFsHuman Mammary FibroblastsStromaExtracellular MatrixMesenchymal CellsFibroblastsMyofibroblastsEndothelial CellsPericytesLeukocytesTumour-associated StromaParacrine Signalsα-smooth Muscle Actin (α-SMA)Activated FibroblastsHigh-grade MalignanciesPoor PrognosesImmunodeficient MiceTumourigenesisSenescence
