चूहे में Sucrose तरस के ऊष्मायन का मूल्यांकन करने के लिए एक सामान्य विधि
Summary
भोजन या दवा बनती cues के लिए जवाब संयम की अवधि के दौरान बढ़ जाती है और यह एक वृद्धि की डूबने व्यवहार करने के लिए संवेदनशीलता के लिए संबंधित हो सकती है. यहाँ चूहों कि आत्म प्रशासित sucrose में इस लालसा के ऊष्मायन "का मूल्यांकन करने के लिए हम एक प्रक्रिया विस्तार.
Abstract
For someone on a food-restricted diet, food craving in response to food-paired cues may serve as a key behavioral transition point between abstinence and relapse to food taking 1. Food craving conceptualized in this way is akin to drug craving in response to drug-paired cues. A rich literature has been developed around understanding the behavioral and neurobiological determinants of drug craving; we and others have been focusing recently on translating techniques from basic addiction research to better understand addiction-like behaviors related to food 2-4.
As done in previous studies of drug craving, we examine sucrose craving behavior by utilizing a rat model of relapse. In this model, rats self-administer either drug or food in sessions over several days. In a session, lever responding delivers the reward along with a tone+light stimulus. Craving behavior is then operationally defined as responding in a subsequent session where the reward is not available. Rats will reliably respond for the tone+light stimulus, likely due to its acquired conditioned reinforcing properties 5. This behavior is sometimes referred to as sucrose seeking or cue reactivity. In the present discussion we will use the term “sucrose craving” to subsume both of these constructs.
In the past decade, we have focused on how the length of time following reward self-administration influences reward craving. Interestingly, rats increase responding for the reward-paired cue over the course of several weeks of a period of forced-abstinence. This “incubation of craving” is observed in rats that have self-administered either food or drugs of abuse 4,6. This time-dependent increase in craving we have identified in the animal model may have great potential relevance to human drug and food addiction behaviors. Here we present a protocol for assessing incubation of sucrose craving in rats. Variants of the procedure will be indicated where craving is assessed as responding for a discrete sucrose-paired cue following extinction of lever pressing within the sucrose self-administration context (Extinction without cues) or as responding for sucrose-paired cues in a general extinction context (Extinction with cues).
Protocol
Discussion
प्रक्रियाओं हम यहाँ वर्णन बहाली प्रक्रियाओं के रूप में पहचाना जा सकता है, हालांकि यह तकनीकी क्यू के लिए लीवर दबाने तरस परीक्षण सत्र के लिए पहले से नहीं बुझा है के रूप में गलत है. एक ठेठ बहाली प्रक्रिया म…
Divulgations
The authors have nothing to disclose.
Acknowledgements
इस पांडुलिपि और वीडियो उत्पादन ड्रग दुर्व्यवहार / स्वास्थ्य अनुदान R15 के राष्ट्रीय संस्थानों DA016285-02, पश्चिमी वाशिंगटन विश्वविद्यालय न्यूरोसाइंस पहल में बायोमेडिकल रिसर्च क्रियाएँ पर राष्ट्रीय संस्थान, और पश्चिमी वाशिंगटन विश्वविद्यालय द्वारा समर्थित किया गया.
Materials
| Item | Company | Catalog number |
| Enrichment Cage | Quality Cage Company | FH-36½T-N |
| Operant Chamber | Med Associates | MED-008-CT-B3 |
| SCH 23390 | Sigma | D054 |
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