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Chapters
- 00:05Title
- 01:14Production of HIV-1 Particles
- 03:04Concentration of HIV-1 Virions by Ultracentrifugation
- 04:30Sucrose Gradient Centrifugation to Isolate Cores
- 06:36Kinetic Assay of HIV-1 Uncoating
- 10:21Conclusion
Uncoating er et væsentligt skridt i den tidlige fase af HIV-1 livscyklus og er defineret som demonteringen af kapsid skallen og frigivelsen af den virale ribonucleoprotein kompleks (vRNP). Her vil vi demonstrere teknikker til at isolere intakte kerner fra HIV-1 virioner og til at kvantificere deres uncoating<em> In vitro</em>.
Tags
In Vitro UncoatingHIV-1 CoresRetrovirusesCapsidLipid EnvelopeLentivirusesCA ProteinHexagon LatticePentamersCone StructureViral Ribonucleoprotein ComplexFusionTarget Cell MembraneCytoplasmDisassemblySoluble CAUncoating ProcessIntracellular LocationTimingAmino-acid SubstitutionsStability Of The CapsidHIV-1 InfectionAssaysQuantitative MethodIsolation Of CoresSedimentationConcentrated VirionsDetergent LayerSucrose Gradient
