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Et eksperimentelt system til Studieinformationen Mechanotransduction i føtale lungeceller
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Chapters
- 00:05Title
- 00:58Coating Culture Plates with Extra-cellular Matrix Proteins
- 02:15Isolation of Fetal Rodent Lung Type II Epithelial Cells
- 05:32Isolation of Fetal Rodent Lung Fibroblasts
- 06:15Experimental System to Provided Mechanical Stimulation to Lung Cells
- 07:42Representative Results from Mechanotransduction Experiments Using Fetal Lung Cells
- 09:15Conclusion
Mekaniske kræfter spiller en central rolle i lungeudvikling og lungebeskadigelse. Her beskriver vi en fremgangsmåde til isolering af gnaver føtal lunge type II epitelceller og fibroblaster, og at udsætte dem for mekanisk stimulation ved hjælp af en<em> In vitro</em> System.
Tags
Experimental SystemMechanotransductionFetal Lung CellsMechanical ForcesLung DevelopmentAlveolar Type II Epithelial CellsPulmonary SurfactantFluid HomeostasisDistal Lung Parenchyma CellsExaggerated StretchMechanical VentilationMolecular MechanismsCellular MechanismsMechanical StimuliLung InjuryIsolation MethodFlexcell Strain UnitIn Vitro SystemMechanical Stimulation
