人乳内动脉（IMA）移植及内支架置入术：人体模型支架内再狭窄的研究开发
1University Heart Center Hamburg,TSI-Lab, Germany, 2Cardiovascular Research Center,University of Hamburg, 3Department of Medicine, Cardiology Division, Pulmonary Hypertension Program,University of Alberta, 4Department of Medicine,Stanford University School of Medicine , 5Department of Biomedical Sciences, Institute of Physiology, Pathophysiology, and Biophysics,University of Veterinary Medicine, Vienna, 6Translumina GmbH,Hechingen, 7Department of Cardiothoracic Surgery,Stanford University School of Medicine
Summary
这个视频显示了一个模型来研究支架使用在人类免疫缺陷鼠模型的船只（IMA）的部署后内膜增生的发展。
Abstract
体内研究模型的临床前研究后内膜增生的血管内支架置入术的发展中的病理生物学和病理生理过程转化为1,2的方法是至关重要的。
常用的动物模型包括小鼠，大鼠，兔，猪3-5。然而，进入临床的设置，这些模型的翻译仍然困难，因为这些生物的进程已经开始研究在动物血管，但以前从未在人类研究模型6,7。在这部影片中，我们展示了一个新的人性化的模型，以克服这种转化的差距。所示的过程，重现性好，操作方便，快速执行，并适合学习内膜增生的发展和多样化支架的适用性。
该视频演示了如何执行，通过移植到免疫缺陷大鼠的血管支架技术，标识作为人类的起源细胞增殖。
Protocol
1。乳内动脉（IMA）准备动脉内皮剥脱2法国福格蒂动脉取栓导管（百特医疗用品，迪尔菲尔德，IL，USA）的通过。拉导管贯穿整个船舶长度的两倍，以确保内皮损伤。 使用的长度为8mm和2.5毫米，直径3毫米（如Translumina，育空支架）的任何人的支架。注意：支架的直径应不超过10％以上的血管直径，以避免前，后支架狭窄。注意：请不要在同一研究中切换支架的长度。 使用合适…
Discussion
虽然在体内研究模型不同的是现有的调查支架植入后内膜增生的发展，这些车型仍然面临着转化的障碍需要克服。此外，大量的动物模型是昂贵和特殊的住房条件以及手术设备是不是所有实验室。
使用人类的IMA研究人类的内膜增生及支架内再狭窄的发展进行了研究之前， 当然在器官培养8,9 原位 。新的人性化的IMA模型构成了翻译的办法来解决支架…
Divulgations
The authors have nothing to disclose.
Acknowledgements
作者感谢她的贡献：克里斯蒂安Pahrmann。特别感谢爱惜康，Norderstedt的，汉堡（德国）提供的缝合材料。
资金
宋佳Schrepfer已收到来自德意志研究联合会（DFG）（SCHR992 / 3 1 SCHR992/4-1）研究补助金。这项工作是由ISHLT沙姆韦事业发展基金2010年和福尔克研究经费（斯坦福大学）的支持。
Materials
| Name of the reagent | Company | Catalog number | Comments |
| 2 French Fogarty catheter | Baxter Healthcare, Deerfield, IL, USA | 120602F | |
| Yukon Stent | Translumina GmbH, Hechingen, Germany | Use the stent of your choice according to your study protocol | |
| RPMI media | Biochrom | Nr.F1275 | |
| heparin | Baxter | 2B0953 | |
| isoflurane | Abbot | B506 | |
| Provo-Iodine | Betadine Puredue Pharma | EAN:5995327165830 | |
| 80% ethanol | Geyer | ETV 80/0500 | |
| Micro clamp | Harvard Apparatus | PY2-61-0186 | |
| Sutures 8-0 | Johnson& Johnson, | 2808G | |
| Sutures 6-0 | Johnson& Johnson, | 1698 H | |
| Carprofen | Feizer Vet | PZN:0110208 | |
| Metamizol | Ratiopharm | ||
| Target retrieval solution, pH9 | Dako | S2368 | |
| Image-iT FX signal enhancer | Invitrogen | I36933 | |
| mouse monoclonal anti-GFP antibody | BD living colors | 632381 | |
| primary antibody diluent | Dako | S3022 | |
| goat-anti-mouse IgG, Alexa Fluor 488 | Invitrogen | A11017 | |
| secondary antibody diluent | Dako | S0809 | |
| rabbit polycolonal anti-smooth muscle α-actin | Abcam, | ab5694 | |
| goat-anti-rabbit IgG, Alexa Fluor 555 | Invitrogen | A21430 | |
| Prolong Gold antifade reagent | Invitrogen | P36930 |
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Tags
Human Internal Mammary Artery (IMA)TransplantationStentingIn-stent RestenosisPreclinical In Vivo Research ModelsIntimal HyperplasiaVessel StentingAnimal ModelsMiceRatsRabbitsPigsClinical SettingsHuman Research ModelsTranslational GapReproducible ProcedureEasy ProcedureFast ProcedureDiverse StentsHuman VesselsImmunodeficient RatsOrigin Of Proliferating Cells
Citer Cet Article
Hua, X., Deuse, T., Michelakis, E. D., Haromy, A., Tsao, P. S., Maegdefessel, L., Erben, R. G., Bergow, C., Behnisch, B. B., Reichenspurner, H., Robbins, R. C., Schrepfer, S. Human Internal Mammary Artery (IMA) Transplantation and Stenting: A Human Model to Study the Development of In-Stent Restenosis. J. Vis. Exp. (63), e3663, doi:10.3791/3663 (2012).