大鼠原位小肠移植
Summary
小肠移植已成为一种公认的不可逆肠衰竭患者的治疗选择。我们作为一个可靠的工具，以解决潜在的免疫和炎症过程的复杂小肠移植大鼠原位小肠移植的实验模型。
Abstract
小肠移植已成为公认的短肠综合征和失败的肠外营养（不可逆肠衰竭）患者的临床治疗选择。在专门的医疗中心，提高操作和管理策略导致了优异的短期和中期患者和移植物存活率，同时提供高品质的生活1,3。不同的是较常见的其他实体器官移植（ 即心脏，肝脏）许多潜在的诱导小肠移植的移植肾功能和免疫学改变的机制尚不完全清楚6,7。发作的急性排斥反应，败血症，慢性移植物功能衰竭的主要障碍仍然较为有利的长期结果，并阻碍更广泛的就业的过程中，尽管越来越多的家庭肠外营养的患者可能受益于这样的移植。小肠包含乘客白细胞的大量的，通常被称为作为肠相关的淋巴系统（GALT）肠道接枝的高免疫原性的原因，这是一部分的一部分。许多共生和病原体在肠道中的存在和靠近解释脓毒症发作的严重程度，一旦接枝粘膜的完整性受到损害（例如，通过抑制）。为了推动该领域的肠和多器官移植产生的数据更可靠和可行的动物模型是必要的。本文结合该模型的可靠性和可行性一旦确立以标准化的方式，可以在底层的复杂的分子，细胞和功能的机制所触发的小肠移植提供了宝贵的见解。我们已经成功地使用超过5年以上的在我们的实验室8-11细化所描述的过程。朱庇特的视频格式是特别有用的，表现出复杂的多项式dure，避免最初的规划，建立原位啮齿动物模型研究小肠移植组的陷阱。
Protocol
1。供体手术应该保持供体鼠禁食24小时（水/葡萄糖溶液的免费访问）。 ，引起吸入异氟醚麻醉，开始以2％的标准雾化器，然后降低到1％后进行剖腹手术。执行脚趾捏了把检查镇静。 刮腹部和清洁皮肤准备3次（Kodan）。然后执行后正中切口皮下给药的镇痛。 后移植物包裹在生理盐水浸泡过的纱布，分离胰腺和升结肠，轻轻地用一个Q-尖端（6倍放大倍率的显微镜下的?…
Representative Results
术后正常移植的动物迅速恢复的过程中，下加热灯约1小时。低温的术后死亡率的一个重要原因，应谨慎地避免内和术后。皮下注射2.5毫升生理盐水加2.5毫升的5％葡萄糖每8个小时，第24小时，术中的流体损失，必须更换。大鼠也应该有术后第一个24小时免费使用葡萄糖溶液（或凝胶饮食）和水区。在此期间，他们应该免费使用标准大鼠饲料和水自由采食恢复正常进食行为。?…
Discussion
最近采用的模型大鼠小肠移植模型中被描述为早在1970ies 5涉及异位小肠移植使用不同的技术13。异位一般程序的优点是更容易的显微外科技术，更容易获得移植物的评估，异位小肠移植有很大的缺点，不考虑的移植小肠的收缩活动功能等方面的多重相互作用黏膜屏障功能提供了广阔的主机的共生和病原体的背景下，原位移植的特点。我们的团队已获得了大量的经验，本文描述的的?…
Divulgations
The authors have nothing to disclose.
Materials
| Name of the reagent | Company | Catalogue number | Comments (optional) |
| University of Wisconsin (UW) solution (ViaSpan) | Bristol-Myers Squibb | ||
| Uro-Nebacetin N solution | Nycomed | 6967855 | |
| Ampicillin | Ratiopharm | ||
| Carprofen (Rimadyl) | Pfizer | ||
| Prolene 10-0 unresorbable suture | Ethicon | ||
| Monocryl 6-0 resorbable suture | Ethicon | ||
| Vicryl 3-0 resorbable suture | Ethicon | ||
| i.v. Catheter G 20 1.1×33 mm | Braun | ||
| i.v. Catheter G 22 0.9×25 mm | Braun | ||
| Kodan Skin Prep | Schülke | ||
| NaCl 0.9% Infusion solution | Braun | ||
| Curved forceps small | FineScienceTools | 11009-13 | |
| Micro forceps curved | AESCULAP | BD 333 | |
| Micro forceps curved | AESCULAP | FD281R | |
| Micro forceps straight 1 | WPI | 5 | |
| Micro forceps straight 2 | WPI | 2 | |
| Micro needle holder | WPI | 14081 | |
| Micro scissors | FineScienceTools | 15006-09 | |
| Micro scalpel | MANI | Ophthalmic knife | |
| Micro clamps | AESCULAP | FB329R |
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Tags
Orthotopic Small Bowel TransplantationRatsShort Gut SyndromeParenteral NutritionIrreversible Intestinal FailureGraft SurvivalQuality Of LifeSolid Organ TransplantationGraft FunctionImmunologic AlterationsAcute RejectionSepsisChronic Graft FailureLong Term OutcomePassenger LeucocytesGut Associated Lymphoid System (GALT)ImmunogenityIntestinal GraftCommensalsPathogensSepsis EpisodesMucosal IntegrityAnimal Models
Citer Cet Article
Kitamura, K., von Websky, M. W., Ohsawa, I., Jaffari, A., Pech, T. C., Vilz, T., Wehner, S., Uemoto, S., Kalff, J. C., Schaefer, N. Orthotopic Small Bowel Transplantation in Rats. J. Vis. Exp. (69), e4102, doi:10.3791/4102 (2012).