无线电遥测系统，以监测大鼠脊髓横断和胚胎神经干细胞移植在心血管功能
Summary
我们提出了一个协议，使用无线电遥测系统，记录在T4脊髓横断大鼠心血管参数八个星期后胚胎脑干神经干细胞移植到病变部位。遥测是一种先进的技术来准确地评估在有意识的自由移动脊髓损伤大鼠的心血管功能。
Abstract
高胸或颈脊髓损伤（SCI），可导致心脑血管功能障碍。为了监测心血管参数，我们植入连接到一个无线发射到大鼠的经历了T4脊髓横断伴或不表达绿色荧光蛋白的胚胎脑干源性神经干细胞移植的股动脉导管。相对于其他方法，例如套管插入或尾套，遥测有利的是连续地监测血压和心脏速率在自由活动动物。它也能够长期的多个数据采集。在脊髓损伤大鼠，针对结直肠扩张奔放的状态，自主神经反射异常在基础的心血管数据被成功记录。此外，前和损伤后的心血管参数可在相同的大鼠进行比较，如果发射器是脊髓横断前注入。所描述的telemet的一个限制Ry的过程是，植入股动脉可影响血液供给到同侧后肢。
Introduction
脊髓损伤（SCI）在较高水平后心血管功能障碍发生。它表现在无序的血压和心脏率在休息，体位性低血压，运动性低血压及自主神经反射异常响应损伤水平以下的1,2感官刺激特征是高血压和压力反射介导的心动过缓发作。这些症状干扰日常生活脊髓损伤患者。因此，为了建立有效的工具，用于在动物中与脊髓损伤和试验性治疗心血管变化的调查是很重要的。
研究在动物的心血管功能，一些技术已经被用于监测血压和心脏速率。中央心血管参数可以通过套管插入和遥测记录，而无创尾袖口可以用来测量外周的血压3。相对于其他方法，远程13655具有的主要优势在于它允许连续记录中自由活动动物和长期监测心血管功能4。在脊髓损伤的动物模型，实验刺激后的变化周血压可能并不大到足以被检测到。因此，合适的心脏监测技术应该被选择用于动物SCI患者。
在本研究中，无线电遥测系统导入后脊髓横断监测成年大鼠心血管功能。大鼠接受的同基因大鼠胚胎第14天（E14）脑干源性神经干细胞（BS-神经干细胞）移植物在病灶部位。大鼠损伤，无移植和天真，没有受伤大鼠作为对照。遥测的步骤包括发送器灭菌并注入（ 图1），记录基底心血管参数，结直肠扩张诱导的反应，并且发送器的清洗和存储。
Protocol
Representative Results
Discussion
传统上，流体填充的套管插入动脉并连接到压力换能器来记录心血管参数在每个动物11的终端快照。连续监测心血管性能很长一段时间，无线电遥测系统被应用在许多实验室。这更精致的工具可以记录在有意识的血压，自由活动动物。相较于充满液体的导管，遥测被认为是一种更先进的技术来准确地评估心血管功能的脊髓损伤动物。虽然在本研究中的发射机被记录之前植入仅1天，它可前?…
Divulgations
The authors have nothing to disclose.
Acknowledgements
The work was supported by grants from NIH/NINDS (NS054883), Craig H. Neilsen Foundation (280072), and the Veterans Administration and Canadian Spinal Research Organization. We thank the Rat Resource and Research Center, University of Missouri, Columbia, Missouri, for providing GFP rats.
Materials
| Table of Specific Materials/Equipment: | |||
| Reagents | Company | Catalogue | Comment |
| Fibrinogen (rat) | Sigma | F6755-25MG | 2 hr at 37oC to dissovle |
| Thrombin (rat) | Sigma | T5772-100UN | Dissovle in 10 mM CaCl2 |
| 1% Terg-A-Zyme | Sigma | Z273287 | Enzymatic solution for telemeter cleaning |
| Fluorogold | Fluorochrome | Dissovle in distilled water and avoid light | |
| Telemeter (PA-C40) | Data Sciences International | ||
| Telementric recording and analysis system | Data Sciences International | Signal stimulator, Data Exchange Matrix, receivers, Ambient pressure reference monitor | |
| Balloon-tipped catheter | Edward Lifesciences | 111F7-P | For colorectal distension |
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