Isolation des droits de cellules primaires de tumeurs du côlon à partir de tissus chirurgicaux et les cultivant directement sur élastique souple substrats pour Traction cytométrie
A protocol is described to extract primary human cells from surgical colon tumor and normal tissues. The isolated cells are then cultured on soft elastic substrates (polyacrylamide hydrogels) functionalized by an extracellular matrix protein, and embedded with fluorescent microbeads. Traction cytometry is performed to assess cellular contractile stresses.