We describe a 3D culture model of the human breast epithelium that is suitable to study hormone action.
The process of mammary epithelial morphogenesis is influenced by hormones. The study of hormone action on the breast epithelium using 2D cultures is limited to cell proliferation and gene expression endpoints. However, in the organism, mammary morphogenesis occurs in a 3D environment. 3D culture systems help bridge the gap between monolayer cell culture (2D) and the complexity of the organism. Herein, we describe a 3D culture model of the human breast epithelium that is suitable to study hormone action. It uses the commercially available hormone-responsive human breast epithelial cell line, T47D, and rat tail collagen type 1 as a matrix. This 3D culture model responds to the main mammotropic hormones: estradiol, progestins and prolactin. The influence of these hormones on epithelial morphogenesis can be observed after 1- or 2-week treatment according to the endpoint. The 3D cultures can be harvested for analysis of epithelial morphogenesis, cell proliferation and gene expression.
不同于标准的2D文化,3D细胞培养替代模型允许的上皮细胞行为生理相关方面,一是类似组织的研究。乳腺三维培养有助于阐明乳腺发育和肿瘤的许多方面。然而,大多数现有的三维培养模型的不适合学习激素的作用,因为用于该任务的上皮细胞缺乏激素受体 表达6,7,9。
在此,我们描述了人类乳腺上皮细胞,适合研究激素作用12的三维培养模型。该模型采用的可商购的激素响应性人乳腺上皮细胞系,T47D 3,11,13,其最初从与乳房的浸润性导管癌从54岁的女性患者获得的胸腔积液而得。我们使用鼠尾胶原1型作为基质。这种3D崇拜URE模型适合于对人乳腺上皮细胞中的三个主要mammotropic激素(雌二醇,promegestone(孕酮类似物),和催乳素)的作用的研究。激素诱导的上皮形态可以定量随时间通过形态分析12进行评估。
适当的接种密度允许保持2周这些3D培养。而此时,结构的发展是足以对上皮形态激素作用的强大的定量评估。凝胶也可以在较早的时间点收获细胞增殖和基因表达分析。此外,这种模式是合适的测试顺序激素治疗的效果;例如,在第一周期间用雌二醇处理和置换在随后一周激素等激素/组合之后。雌激素化合物和抗雌激素,例如ICI 182,780的效果,也可以苹果塞进袋子使用这种三维培养模型12死亡。
Here, we describe a hormone-sensitive 3D culture model to test the action of hormones on breast epithelium. The response to hormones can be assessed at the tissue morphology, cell proliferation and gene expression levels 12. One limitation of this technique is that visualization during the culture period is restricted to light microscopy since the cultures are grown in a plastic bottom plate. The 3D culture system could be adapted to glass bottom plates to allow for live imaging of the cultures 1.</…
The authors have nothing to disclose.
我们非常赞赏谢丽尔Schaeberle社论贡献。这项研究是由雅芳资助#02-2009-093和02-2011-095和NIEHS / NIH ES 08314至AMS支持。内容完全是作者的责任,并不一定代表环境健康科学研究所或美国国立卫生研究院的官方意见。
12-well Tissue Culture Plates (Falcon) | Fisher Scientific | 08-772-29 | |
15 ml polystyrene conical Tubes | Fisher Scientific | 14-959-49D | |
Activated Charcoal | Sigma | C-5510 | |
Carmine Alum | Sigma | C1022-100G | |
Collagenase, Type 3 | Worthington | S0C11784 | |
Confocal Microscope | Zeiss | LSM510 | Equiped with HeNe 633nm laser |
Dextran T-70 | Abersham/Pharmacia | 17-0280-01 | |
DMEM/F-12, HEPES, no phenol red | Life Technologies | 11339-021 | Phenol red-free media for hormone use |
DMEM, low glucose, pyruvate, no glutamine, no phenol red | Life Technologies | 11054-020 | Phenol red-free media for hormone use |
17-β-Estradiol | EMD Millipore | 3301 | Dissolved in Ethanol |
Ethanol | Koptec | V1001 | |
Fetal Bovine Serum | Hyclone | SH30070.03 | For use with hormones, must be Charcoal Dextran stripped |
Filters (115ml) | Nalgene | 380-0080, 245-0045, 120-0020 | 0.88, 0.45, 0.20 micron, respectively |
Formalin, 10% | Fisher Scientific | SF93-20 | |
L-Glutamine (200 mM) | Life Technologies | 25030-081 | |
ICI 182,780 (fulvestrant) | Sigma Aldrich | I4409-25MG | Dissolved in DMSO |
Microtome | Leica | RM2155 | |
Tissue embedding media | McCormick Scientific | 39502004 | |
Penicillin | Sigma | 7794-10MU | Dissolved in 10 ml of distilled deionized water |
Permount | Fisher Scientific | SP15-500 | |
Phosphate Buffered Saline pH 7.4 | Sigma Aldrich | P3813-10PAK | |
Prolactin | Sigma Aldrich | L4021-50UG | Dissolved in distilled deionized water |
Promegestone | Perkin Elmer | NLP004005MG | Dissolved in Ethanol |
Rat-Tail Collagen | Corning | 354236 | Lots may contain varying concentrations, note accordingly |
Scalpel | Miltex | 4311 | |
Semi-enclosed Benchtop Tissue Processor | Leica | TP1020 | |
Sodium Hydroxide | Sigma Aldrich | S5881 | Prepare 1N NaOH stock |
StaticMaster Anti-static brush | Amstat | C3500 | |
Stripette Serological Pipettes | Corning | 4101 | |
T-25 flasks | Corning | 430168 | |
Tissue Cassettes | Fisher Scientific | 15-200-403E | |
Wheaton Vials, Glass, 20mL | Fisher Scientific | 03-341-25D | |
Xylene | VWR | 95057-822 |