ट्रांसवर्स महाधमनी कसना के माउस मॉडल में कार्डिएक रूपात्मक के आकलन और कार्यात्मक परिवर्तन एचोकर्दिओग्रफिक इमेजिंग द्वारा
Summary
इस प्रोटोकॉल के लक्ष्य noninvasively, अनुप्रस्थ महाधमनी कसना द्वारा बनाई हृदय रोग का एक माउस मॉडल में हृदय की संरचनात्मक और कार्यात्मक परिवर्तन का आकलन करने के लिए बी और एम मोड इकोकार्डियोग्राफी और रंग / पल्स लहर डॉपलर इमेजिंग का उपयोग है।
Abstract
Transverse aortic constriction (TAC) in mice has been used as a valuable model to study mechanisms of cardiac hypertrophy and heart failure1. A reliable noninvasive method is essential to assess real-time cardiac morphological and functional changes in animal models of heart disease. Transthoracic echocardiography represents an important tool for noninvasive assessment of cardiac structure and function2. Here we used a high-resolution ultrasound imaging system to monitor myocardial remodeling and heart failure progression over time in a mouse model of TAC. B-mode, M-mode, and Doppler imaging were used to precisely assess cardiac hypertrophy, ventricular dilatation, and functional deterioration in mice following TAC. Color and pulse wave (PW) Doppler imaging was used to noninvasively measure pressure gradient across the aortic constriction created by TAC and to assess transmitral blood flow in mice. Thus transthoracic echocardiographic imaging provides comprehensive noninvasive measurements of cardiac dimensions and function in mouse models of heart disease.
Introduction
Mouse models of heart disease, such as TAC and myocardial infarction (MI), have been proven to be valuable to study disease mechanisms as well as to develop novel therapeutic strategies3. TAC initially induces compensatory hypertrophy, but prolonged pressure overload leads to cardiac dilatation and heart failure4. The tightness of the aortic constriction directly determines the degree of cardiac hypertrophy and its transition to heart failure. Noninvasive and reliable measurement of pressure gradient across the aortic constriction is essential for the success of these studies. Doppler imaging has been used to assess pressure gradient produced by TAC5, which is a noninvasive alternative for catheter-based pressure measurement.
Echocardiography has been widely used to noninvasively measure cardiac morphology as well as systolic and diastolic function in mice6-8. Two-dimensional B-mode imaging is used to detect abnormal movements or structural changes of the heart. One-dimensional M-mode imaging is used for quantification of cardiac dimensions and contractility. Color and PW Doppler imaging has recently been used on rodent ultrasound, which has broad applications for echocardiography, including measurement of flow directionality and velocity, as well as systolic and diastolic performance9.
Longitudinal real-time monitoring of cardiac function using echocardiography in B-mode, M-mode, color and PW Doppler mode provides comprehensive assessment of cardiac structure and function in mice under physiological and pathological conditions. Here we provide a detailed description of the use of echocardiographic imaging to monitor dynamic cardiac morphological and functional changes in mice following TAC or sham surgery.
Protocol
Representative Results
Discussion
इकोकार्डियोग्राफी व्यापक रूप से हृदय रोग 2,6 के कृंतक मॉडल में हृदय समारोह का आकलन करने के लिए इस्तेमाल किया गया है। इस तरह के दबाव मात्रा पाश माप 11 के रूप में आक्रामक या टर्मिनल के तरीके की तुलना…
Divulgations
The authors have nothing to disclose.
Acknowledgements
The authors have nothing to disclose.
Materials
| Anesthesia equipment | Harvard Apparatus, 84 October Hill Road Holliston, MA |
723015 | |
| Vevo 2100 Imaging System | VisualSonics Inc., 3080 Yonge Street Suite 6100, Box 66, Toronto, Ontario, Canada | Vevo 2100 | |
| Aquasonic ultrasound gel | Parker Laboratories, 286 Eldridge Rd, Fairfield, NJ | 03-50 | |
| Isoflurane | Piramal Healthcare, Inc, 3950 Schelden Circle Bethlehem, PA |
NDC 66794-017-25 | |
| F/air anesthesia gas filter unit | A.M. Bickford, Inc, 12318 Big Tree Rd, Wales Center, NY | 80120 |
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