Her introducerer vi en metode til evaluering af transpedikulære skruer ved hjælp af en in vivo porcine lændehvirvelsøjle model.
Pedicle skruefiksering er guldstandarden til behandling af rygsygdomme. Men mange undersøgelser har rapporteret spørgsmålet om løsnelse af pedikelskruer efter rygsmerter, hvilket er et alvorligt problem. For at løse dette problem er forskellige typer af pedicle skruer undersøgt for at identificere dem med god fikseringsstyrke og osseointegration i rygsøjleben. Den svære rygsøjle er et godt alternativ til den menneskelige rygsøjle i vurderingen af pedicle skruer på grund af den anatomiske størrelse, mekaniske egenskaber og omkostninger. Selvom flere undersøgelser har rapporteret, at pedicle skruer er effektive i svinemodellen, har ingen undersøgelser beskrevet detaljerede protokoller til evaluering af en pedicle skrue ved hjælp af svinemodellen. Her beskriver vi en detaljeret metode til evaluering af transpedikulære skruer ved hjælp af en in vivo porcine lændehvirvelsøjle model. De tekniske detaljer for anæstesi, rygsmerter og høst, der er tilvejebragt her, vil lette med evalueringen af tRanspedikulær skruefikseringsmodel.
Transpedicular screw fixation is a gold-standard treatment for degenerative lumbar spine and bursting fracture because it involves three columns of the spine and achieves stabilization1,2. However, most patients who undergo such surgery also have osteoporosis3,4. Many studies have evaluated the fixation strength and the osseointegration status of transpedicular screws, because the loosening of pedicle screws currently in use has been reported in patients with osteoporosis5,6.
The porcine spine is similar to the human spine in terms of size. It is less expensive compared to a primate model7. Furthermore, an in vivo mechanical study has demonstrated that the quadruped porcine spine is essentially loaded in the same way as that of the human spine8, which is why many researchers use porcine spines for studies on the prevention of pedicle screw loosening. However, it takes several months to study pedicle screws in the porcine spine because identifying the long-term stability of pedicle screws takes times. In order to compare different types of screws in the vertebral body, it is necessary to insert the screws in similar positions. Therefore, researchers should be well-acquainted with proper anesthesia techniques, standardized surgical protocols, and harvest procedures before performing any experiments. Here, we describe a detailed method for anesthesia, surgery, and harvest for the evaluation of pedicle screw fixation using a porcine spine model, including ex vivo imaging, histology, and strength testing.
Evalueringen af transpedikulære skruer i svinesyggen kræver meget tid og kræfter. For det første er miniatyret et stort dyr. For dyrepleje og anæstesi har forskeren brug for en specialprotokol. For det andet skal kirurgi opretholde et miljø, der ligner det humane kirurgi. Formålet med at evaluere pedicle skruer i svinesygdom er at udvikle en effektiv skrue, der kan anvendes på mennesker. For det tredje kræver evaluering af transpedikulære skrues langsigtede stabilitet omkring tre måneder efter rygsøjle…
The authors have nothing to disclose.
Denne undersøgelse blev støttet af et tilskud (CNUH-BRI-2012-02-005) finansieret af Biomedicinsk Forskningsinstitut i Chonbuk National University Hospital (CNUH-BRI), Republikken Korea.
Miniature pig | OrientBio | ||
Atropine | Jeil pharmaceutical | A04900241 | Anesthesia |
Over-the needle plastic catheter | BD | REF382412 | Maintenance of IV line |
Ketamine | Yuhan | A04502441 | Anesthesia |
Xylazine | Bayer Korea | A00800071 | Anesthesia |
Laryngoscope | Karl storz | Intubation | |
Endotracheal tube | Covidien | Intubation | |
Isoflurane | JW pharmaceutical Co | A02104781 | Anesthesia |
Eye ointment | Hanlim pharma | A37851721 | Protection of pig's eye |
Cefazolin | Donga pharma | A01503951 | Antibiotics |
Saline | JW pharmaceutical Co | A02151392 | Maintenance of fluid homeostasis |
Fentanyl | Hana pharm | C03200032 | Pain control |
Enrofloxacin | Bayer | 93106-60-6 | Antibiotics |
Morphine | Myungmoon pharma | C03700091 | Pain control |
Meloxicam | Boehringer Ingelheim | A07600711 | Antibiotics |
Povidone-iodine | Hyundai pharma | Wound dressing | |
Scalpel blade size 15 | Braun | I1 BB515 | Skin incision |
Cobb elevator | Codman | 65-2546 | Dissection of muscle |
Burr | Medtronic | Making of starting point of screw | |
Rongeur | Aesculap | FO515R | Making of starting point of screw |
Guide pin (K-wire) | CE | 01067803 | Guidance of screw trajectory |
C-arm | GE | OEC 9800 plus | Guidance of screw trajectory |
Portable X-ray | Siemens | Mobile XP hybrid | Guidance of screw trajectory |
Pedicle probe | OtisBiotech | SPI-02-01 | Guidance of screw trajectory |
Pedicle sounding device | OtisBiotech | SPI-03-01 | Guidance of screw trajectory |
Pedicle screw | OtisBiotech | MS-40025 | |
Posterior fixator systems | OtisBiotech | ||
Rod | OtisBiotech | ROD-60140 | Rigid fixation between screws |
Universal handle | OtisBiotech | SPI-08-01 | To fix the screws to the rod |
Straight socket wrench | OtisBiotech | SPI-06-01 | To fix the screws to the rod |
counter torque wrench | OtisBiotech | SPI-07-01 | To fix the screws to the rod |
Bulb irrigation syringe | Hyupsug medical | HS-IR-140 | Irrigation |
Silicone drain | Sewon medical | 2205-006 | To drain the fluid at the surgical site |
3.0 metric absorbable suture | Ethicon | BA1673H | Muscle suture |
2.0 metric nonabsorbable nylon suture | Ethicon | W1626T | Skin suture |
Gauze | Kingphar Korea | KP120-06 | |
Pentobarbital | Hanlim pharma | 645301221 | Euthanasia |
Oscillating saw | Zimmer | Harvest spine | |
Tower forceps | Aesculap | BF461R | Harvest spine |