Summary

Erratum: Isolation of Human Hepatocytes by a Two-step Collagenase Perfusion Procedure

Published: July 01, 2016

Summary

A correction was made to: Isolation of Human Hepatocytes by a Two-step Collagenase Perfusion Procedure.

The Final Concentration of EGTA in Solution 2 and the Final Concentration of Calcium chloride dihydrate in solutions 3 and 4 have been updated in Table 1.

Abstract

A correction was made to: Isolation of Human Hepatocytes by a Two-step Collagenase Perfusion Procedure

The changes listed below have been made to Table 1.

1). In the recipe for Solution 2, the Final Concentration of EGTA has been changed from:

EGTA  0.1mM

to:

EGTA  1mM

2). In the recipes for Solution 3 and 4, the Final Concentration of Calcium chloride dihydrate has been changed from:

Calcium chloride dihydrate  0.5µM

to:

Calcium chloride dihydrate  5mM

Protocol

A correction was made to: Isolation of Human Hepatocytes by a Two-step Collagenase Perfusion Procedure

The changes listed below have been made to Table 1.

1). In the recipe for Solution 2, the Final Concentration of EGTA has been changed from:

EGTA  0.1mM

to:

EGTA  1mM

2). In the recipes for Solution 3 and 4, the Final Concentration of Calcium chloride dihydrate has been changed from:

Calcium chloride dihydrate  0.5µM

to:

Calcium chloride dihydrate  5mM

Divulgations

The authors have nothing to disclose.

Tags

Citer Cet Article
Erratum: Isolation of Human Hepatocytes by a Two-step Collagenase Perfusion Procedure. J. Vis. Exp. (113), e5804, doi: (2016).

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