Journal
/
/
通过Sialoglycan的代谢标记识别主要神经干细胞和祖细胞的细胞表面标记
Journal JoVE
Neurosciences
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Neurosciences
Identifying Cell Surface Markers of Primary Neural Stem and Progenitor Cells by Metabolic Labeling of Sialoglycan
DOI:

11:39 min

September 07, 2019

, ,

Chapitres

  • 00:05Titre
  • 01:16Preparation of Mouse Endothelial Culture in Permeable Support Inserts
  • 01:55Set-up of Neural-endothelial Co-culture and Ac4ManNAz Labeling System
  • 03:25Immunofluorescent Staining of Sialoglycoproteins in Expanded Primary NSPCs and Differentiated Neurons
  • 05:25Purification of Sialoglycoproteins from Expanded Primary NSPCs and Differentiated Neurons
  • 08:45Results: Identifying Cell Surface Markers by Metabolic Labeling of Sialoglycan
  • 10:47Conclusion

Summary

Traduction automatique

这里介绍的是一个协议,结合了体外神经内皮共培养系统和代谢结合的异形理念功能组,以扩大主神经干和祖细胞,并标记其表面用于细胞表面标记的成像或质谱分析的紫锥蛋白。

Vidéos Connexes

Read Article