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由上皮细胞和原发免疫细胞组成的多细胞人类Alveal模型，用于危险评估

Journal JoVE
Biologie

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Journal JoVE Biologie

Multicellular Human Alveolar Model Composed of Epithelial Cells and Primary Immune Cells for Hazard Assessment

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由上皮细胞和原发免疫细胞组成的多细胞人类Alveal模型，用于危险评估

DOI:

10.3791/61090-v

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09:27 min

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May 06, 2020

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Hana Barosova*¹, Barbara Drasler*¹, Alke Petri-Fink, Barbara Rothen-Rutishauser

¹Adolphe Merkle Institute,University of Fribourg

Chapitres

00:04Introduction
01:11Peripheral Blood Monocytes (PBM) Isolation
03:07CD14⁺ Cell Selection
04:00PBM Seeding and Differentiation
04:46Human Alveolar Epithelial Tissue Triple Cell Coculture Model
05:22MDDC and MDM Seeding
07:03Coculture Model Transfer to Air-Liquid Interface
07:30Results: Representative Multicellular Human Alveolar Model Hazard Assessment
08:52Conclusion

Summary

Traduction automatique

这里介绍的是原发性人类血液单细胞分离的协议，以及它们分化成巨噬细胞和树突状细胞，并组装上皮细胞成多细胞人类肺模型。比较了由免疫细胞组成的生物反应，这些细胞与新鲜分离或解冻的单核细胞不同，在接触刺激性刺激时。

Tags

Multicellular Human Alveolar Model Epithelial Cells Primary Immune Cells Hazard Assessment Acute Immune Response Nanomaterials Drugs Monocytes Immune Communication Cell Culture Peripheral Blood Mononuclear Cells Density Gradient Centrifugation

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