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Journal
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Neuroscience
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Ex Vivo
神経生理学のためのラット坐骨神経の調製
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Preparation of Rat Sciatic Nerve for
Ex Vivo
Neurophysiology
Please note that all translations are automatically generated.
Click here for the English version.
Ex Vivo
神経生理学のためのラット坐骨神経の調製
DOI:
10.3791/63838-v
•
09:09 min
•
July 12, 2022
•
Adrien Rapeaux
2,3,4
,
Omaer Syed
,
Estelle Cuttaz
,
Christopher A. R. Chapman
,
Rylie A. Green
,
Timothy G. Constandinou
2,3,4
1
Department of Electrical and Electronic Engineering
,
Imperial College London
,
2
Centre for Bioinspired Technology
,
Imperial College London
,
3
Care Research and Technology (CR&T)
,
Imperial College London and the University of Surrey
,
4
Dementia Institute (UK DRI)
,
5
Department of Bioengineering
,
Imperial College London
Chapters
00:04
Introduction
00:38
Dissection Protocol
02:16
Nerve Cleaning Procedure
03:21
Equipment Setup
05:28
Electrode Implantation on the Nerve in the Bath
06:20
Stimulation and Recording
07:31
Results: Ex Vivo Neurophysiology of Rat Whole Sciatic Nerve Tissue
08:19
Conclusion
Summary
Automatic Translation
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Automatic Translation
このプロトコールは、
エキソビボ
電気生理学的刺激および環境的に調節された2区画の灌流生理食塩水浴中での記録のためのラット全坐骨神経組織の調製を記載している。
Tags
Rat
Sciatic Nerve
Ex Vivo Neurophysiology
Nerve Block
High Frequency/high Amplitude Current
In Vivo Electrophysiology
Euthanization
Dissection
Krebs-Henseleit Buffer
MKHB
Hemostats
Spinal Cleft
Forceps
Scissors
Petri Dish
Sutures
Article
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