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Cytotoksicitetsanalyser med zebrafiskcellelinjer
JoVE Journal
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Cytotoxicity Assays with Zebrafish Cell Lines
DOI:

08:22 min

January 06, 2023

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Chapters

  • 00:04Introduction
  • 01:02Plating the Cells in 96-Well Plates
  • 02:01Exposing the Cells to Test Chemicals
  • 03:19Performing the Cytotoxicity Assays
  • 05:51Results: Calculation of Cell Viability for Different Test Chemicals
  • 07:39Conclusion

Summary

Automatic Translation

Denne protokol præsenterer almindeligt anvendte cytotoksicitetsassays (Alamar Blue [AB], CFDA-AM, Neutral Red og MTT-assays) tilpasset til vurdering af cytotoksicitet i zebrafiskembryo (ZEM2S) og lever (ZFL) cellelinjer i 96-brøndplader.

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