Summary
The eggs of Xenopus laevis intact, lysed, and/or fractionated are useful for a wide variety of experiments. This protocol shows how to induce egg laying, collect and dejelly the eggs, and sort the eggs to remove any damaged eggs.
Abstract
The eggs of Xenopus laevis intact, lysed, and/or fractionated are useful for a wide variety of experiments. This protocol shows how to induce egg laying, collect and dejelly the eggs, and sort the eggs to remove any damaged eggs.
Protocol
The complete text protocol for this experimental approach is available in Current Protocols in Cell Biology.
Divulgations
The authors have nothing to disclose.
Citer Cet Article
Cross, M. K., Powers, M. Obtaining Eggs from Xenopus laevis Females. J. Vis. Exp. (18), e890, doi:10.3791/890 (2008).