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Analysis of Physiologic E-Selectin-Mediated Leukocyte Rolling on Microvascular Endothelium
Journal JoVE
Biologie
This content is Free Access.
Journal JoVE Biologie
Analysis of Physiologic E-Selectin-Mediated Leukocyte Rolling on Microvascular Endothelium
DOI:

14:16 min

February 11, 2009

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Chapitres

  • 00:00Titre
  • 00:48Introduction
  • 01:36Preparation of hBMEC Monolayer on Petri Dishes
  • 03:03Preparation of Human Hematopoietic Progenitor KG1a Cells
  • 03:44Preparation of Microscope, Parallel-Plate Flow Chamber and Syringe Pump
  • 06:26Capturing Leukocyte Rolling Events using NIS Elements
  • 09:21Dependence of Terminal Sialylation and Surface Glycoprotein for E-selectin-Mediated KG1a Cell Rolling
  • 10:43Analysis of NIS-Elements-Captured KG1a Cells and Graphing of KG1a Cell Rolling Data
  • 12:27Representative Results
  • 13:35Conclusion

Summary

Traduction automatique

This report provides a visual depiction of parallel-plate flow chamber analysis for studying leukocyte endothelial interactions under physiologic shear stress. This method is particularly useful for investigating the role of endothelial (E)-selectin and leukocyte E-selectin ligands that trigger leukocyte rolling on endothelial cell surfaces.

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