Journal
/
Biologie
/
The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation
Journal JoVE
Biologie
This content is Free Access.
Journal JoVE Biologie
The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation

The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation

DOI:
10.3791/1026-v

•

08:15 min

•

January 06, 2009

•

, ,
1Gene Expression Division,Bio-Rad Laboratories, Inc

Chapitres

  • 00:00Titre
  • 00:08Introduction
  • 00:43Harvesting Bone Marrow From Femurs and Tibiae
  • 02:07Establishing Cultures
  • 04:33Electroporating Cells
  • 07:13Transfection Results
  • 07:53Conclusion

Summary

Traduction automatique

Traduction automatique

This procedure describes how to establish primary hematopoietic cell cultures from murine bone marrow and is followed by transfection using the Gene Pulser MXCell electroporation system.

Tags

Primary Hematopoietic Cell CulturesMurine Bone MarrowElectroporationPlasmid DNASiRNAGene Pulser MXcell Electroporation SystemGene Pulser Electroporation BufferTransfectionNucleic AcidsMammalian CellsDifficult-to-transfect CellsStem CellsVideo DemonstrationFemurTibia

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/1026?article_type=v

Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells
check_url/fr/1026?article_type=v

Primary Neuronal Cultures from the Brains of Late Stage Drosophila Pupae
check_url/fr/1026?article_type=v

Preparation of Neuronal Cultures from Midgastrula Stage Drosophila Embryos
check_url/fr/1026?article_type=v

Electroporation of Mycobacteria
check_url/fr/1026?article_type=v

Primary Dissociated Midbrain Dopamine Cell Cultures from Rodent Neonates
check_url/fr/1026?article_type=v

Homing of Hematopoietic Cells to the Bone Marrow
check_url/fr/1026?article_type=v

Primary Culture and Plasmid Electroporation of the Murine Organ of Corti.
check_url/fr/1026?article_type=v

Primary Cell Cultures from Drosophila Gastrula Embryos
check_url/fr/1026?article_type=v

Preparation of Primary Myogenic Precursor Cell/Myoblast Cultures from Basal Vertebrate Lineages
check_url/fr/1026?article_type=v

Osteoclast Derivation from Mouse Bone Marrow

Read Article