Journal
/
Biologie
/
Preparation of Single-Cell Suspensions from Mouse Spleen with the gentleMACS Dissociator
Journal JoVE
Biologie
This content is Free Access.
Journal JoVE Biologie
Preparation of Single-Cell Suspensions from Mouse Spleen with the gentleMACS Dissociator

Preparation of Single-Cell Suspensions from Mouse Spleen with the gentleMACS Dissociator

DOI:
10.3791/1029-v

•

05:18 min

•

December 11, 2008

•

, , , ,
1Miltenyi Biotec,GmbH

Chapitres

  • 00:00Titre
  • 00:26Introduction
  • 00:58Introduction to Equipment
  • 01:55Dissociating the spleen using the gentleMACS Dissociator
  • 03:42Filtration of dissociated spleen
  • 04:49Conclusion

Summary

Traduction automatique

Traduction automatique

This video describes a simple, time-saving technique for automated tissue dissociation using the gentleMACS Dissociator to prepare single-cell suspensions of mouse splenocytes.

Tags

Single-Cell SuspensionsMouse SpleenGentleMACS DissociatorExperimentsCell SeparationCell AnalysisCell CultureManual DissociationControlled ConditionsReproducible ConditionsUser-safetyVideo TutorialAutomated DeviceViable Cell SuspensionsFilteringCentrifugationResuspension

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

Standardized Preparation of Single-Cell Suspensions from Mouse Lung Tissue using the gentleMACS Dissociator

Generation of Single-Cell Suspensions from Mouse Neural Tissue

Single-cell Suction Recordings from Mouse Cone Photoreceptors

Generation of Mice Derived from Induced Pluripotent Stem Cells

Simple and Efficient Technique for the Preparation of Testicular Cell Suspensions

Isolation of Endothelial Cells from the Lumen of Mouse Carotid Arteries for Single-Cell Multi-Omics Experiments

Single-Cell Suspension Preparation from Nile Tilapia Intestine for Single-Cell Sequencing

Real-Time Detection of Reactive Oxygen Species Production in Immune Response in Rice with a Chemiluminescence Assay

Isolation and Transcriptome Analysis of Plant Cell Types

Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

Read Article