Journal
/
Biologie
/
Single Molecule Methods for Monitoring Changes in Bilayer Elastic Properties
Journal JoVE
Biologie
This content is Free Access.
Journal JoVE Biologie
Single Molecule Methods for Monitoring Changes in Bilayer Elastic Properties

Single Molecule Methods for Monitoring Changes in Bilayer Elastic Properties

DOI:
10.3791/1032-v

•

12:20 min

•

November 03, 2008

•

, , ,
1Department of Physiology and Biophysics,Weill Cornell Medical College, 2Department of Physiology and Biophysics,Weill Cornell Medical College of Cornell University

Chapitres

  • 00:00Titre
  • 00:12Introduction
  • 01:18Preparing for Bilayer Experiments
  • 03:44Forming a Membrane
  • 04:43Gramicidin Channels
  • 05:43Recording the Control Current Traces
  • 06:38Recording the Experimental Current Traces
  • 07:41Data Analysis
  • 11:54Conclusion

Summary

Traduction automatique

Traduction automatique

Membrane protein function is regulated by the cell membrane lipid composition. This video-article details how to form a patch using bilayer patch electrodes, as well as how to use gramicidin channels as reporters of altered membrane properties.

Tags

Single Molecule MethodsMonitoringChangesBilayer Elastic PropertiesMembrane Protein FunctionCell Membrane Lipid CompositionLipid-protein InteractionsLipid Bilayer-protein InteractionsPharmacological ResearchPharmaceuticalsLipid Bilayer Material PropertiesAltered Membrane Protein FunctionGramicidin ChannelsConformational ChangesGramicidin SubunitsProperties Of The LipidGramicidin Channel CurrentReporter Compounds

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/1032?article_type=v

Visualizing Single-molecule DNA Replication with Fluorescence Microscopy
check_url/fr/1032?article_type=v

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
check_url/fr/1032?article_type=v

Single-Molecule Imaging of Nuclear Transport
check_url/fr/1032?article_type=v

Gramicidin-based Fluorescence Assay; for Determining Small Molecules Potential for Modifying Lipid Bilayer Properties
check_url/fr/1032?article_type=v

Monitoring Equilibrium Changes in RNA Structure by ‘Peroxidative’ and ‘Oxidative’ Hydroxyl Radical Footprinting
check_url/fr/1032?article_type=v

Long-term Lethal Toxicity Test with the Crustacean Artemia franciscana
check_url/fr/1032?article_type=v

AFM-based Mapping of the Elastic Properties of Cell Walls: at Tissue, Cellular, and Subcellular Resolutions
check_url/fr/1032?article_type=v

Studying DNA Looping by Single-Molecule FRET
check_url/fr/1032?article_type=v

A Proteoliposome-Based Efflux Assay to Determine Single-molecule Properties of Cl– Channels and Transporters
check_url/fr/1032?article_type=v

Xenopus Oocytes: Optimized Methods for Microinjection, Removal of Follicular Cell Layers, and Fast Solution Changes in Electrophysiological Experiments

Read Article