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Photo-Induced Cross-Linking of Unmodified Proteins (PICUP) Applied to Amyloidogenic Peptides
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Journal JoVE Biologie
Photo-Induced Cross-Linking of Unmodified Proteins (PICUP) Applied to Amyloidogenic Peptides

Photo-Induced Cross-Linking of Unmodified Proteins (PICUP) Applied to Amyloidogenic Peptides

DOI:
10.3791/1071-v

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08:40 min

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January 12, 2009

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, ,
1Department of Neurology, David Geffen School of Medicine,University of California, Los Angeles, 2Brain Research Institute, Molecular Biology Institute,University of California, Los Angeles, 3Department of Neurology,University of California, Los Angeles

Chapitres

  • 00:00Titre
  • 00:53Introduction
  • 01:50Preparing The Peptides
  • 03:32Solubilizing and Photo-Cross-Linking the HFIP-Treated Peptide
  • 06:22Representative Result
  • 07:54Conclusion

Summary

Traduction automatique

Traduction automatique

Photo-induced cross-linking of unmodified proteins (PICUP) allows characterization of oligomer size distribution in metastable protein mixtures. We demonstrate application of PICUP to three representative amyloidogenic peptides the 40- and 42-residue forms of amyloid β-protein, and calcitonin, and a control peptide growth-hormone releasing factor.

Tags

Photo-Induced Cross-Linking Of Unmodified ProteinsPICUPAmyloidogenic PeptidesProtein Misfolding DiseasesAlzheimer’s DiseaseParkinson’s DiseaseHuntington’s DiseaseHereditary Amyotrophic Lateral SclerosisType 2 DiabetesOligomer Size DistributionClassical MethodsElectrophoresisChromatographyFluorescenceDynamic Light ScatteringMetastable MixturesCovalent Cross-linkingSodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE)Size-exclusion Chromatography (SEC)VisualizationQuantitative AnalysisAssembly MonitoringSequence ModificationsOligomerization Mechanism

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