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Chapitres
- 00:00Titre
- 00:58Introduction
- 01:27Disruption of the Influenza A Virion
- 02:53Glycerol Gradient Sediment Velocity Centrifugation
- 04:18Analysis of the Glycerol Gradient Fractions by SDS Polyacrylamide Gel Electrophoresis
- 05:40Concentration of the vRNP Fractions
- 06:39Negative Staining of vRNPs
- 08:25Representative Results/ Outcome
- 09:05Conclusion
The genome of the influenza A virus consists of eight separate complexes of RNA and proteins, termed viral ribonucleoprotein complexes (vRNPs). This paper describes the glycerol gradient purification and transmission electron microscopy visualization of influenza A vRNPs.
Tags
PurificationVisualizationInfluenza AViral Ribonucleoprotein ComplexesVRNPsViral EnvelopeNuclear ImportReplicationIsolated VRNPsProcédureDisruptionDetergentsGlycerol GradientVelocity SedimentationSDS-PAGECoomassie Blue StainingPeak FractionsConcentrationCentrifugationIntegrity VerificationTransmission Electron Microscopy
