Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Chapitres
- 00:05Titre
- 00:48Introduction
- 01:21Facility Setup
- 03:08Pancreas Preparation and Perfusion
- 06:15Pancreas Digestion
- 07:24Tissue Collection
- 10:24Representative Results: Stained Pancreatic Cells
- 10:44Conclusion
Achieving high quality and appropriate quantity of human islets is one of the prominent prerequisites for successful islet transplantation. In this video, we describe step by step the procedures for human pancreatic islet isolation (part I: digestion and collection of pancreatic tissue) using a modified automated method.
Tags
Human Pancreatic Islet IsolationDigestionCollectionType 1 DiabetesGlucose ControlInsulin TherapyIslet TransplantationDonor CharacteristicsOrgan ProcurementPreservationIsolation ProtocolPurification GradientCold Preservation SolutionsCell Isolation LaboratoryUIC MethodRicordi Et Al MethodEnzyme Solution
