Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Chapitres
- 00:00Titre
- 00:47Introduction
- 01:28Mounting Zebrafish Embryos for Long-Term Imaging
- 03:25Two-photon axotomy using a custom built two-photon microscope with a Chameleon Ti-Sapphire laser
- 04:42Two-photon axotomy on Zeiss 510 confocal/ two-photon microscope
- 06:52Confocal time-lapse imaging on Zeiss LSM 510
- 10:56Outcome and Results
- 11:44Conclusion
Tags
Two-photon AxotomyTime-lapse Confocal ImagingZebrafish EmbryosCellular Mechanisms Of DevelopmentLong-term ImagingAutomated Image CaptureConfocal MicroscopePeripheral Sensory AxonsFemtosecond LaserTwo-photon MicroscopeGFP ExpressionSensory Neuron RegenerationAnesthetized EmbryosAgarose PositioningImaging Chamber
