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Chapitres
- 00:00Titre
- 00:56Introduction
- 01:25‘Inside-Out’ Tissue Preparation
- 04:29Neuronal Bouton Labeling with Fluorescently Labeled Primary Antibody Against HRP
- 05:23Perisynaptic Space Labeling with Dextran Fluorophore Conjugate
- 06:12Mounting the Tissue for Visualization Under a Confocal Microscope
- 07:23Representative Results
- 10:17Conclusion
Descrevemos as características estruturais das sinapses Glia-neuromuscular em um romance de preparação dos tecidos dentro para fora-de larvas de moscas ao vivo usando corantes fluorescentes com microscopia confocal. Nós rotulados terminais de neurônios fluorescentes viver com anticorpos primários para HRP, e também visualizar o espaço perisynaptic com Dextranos fluorescente.
Tags
VisualizingLiveDrosophilaGlial-neuromuscular JunctionFluorescent DyesGFP Labeled Glial StructuresDeveloping Larval Fly Neuromuscular SynapseLive Glial-nerve-muscle SynapsesOptical PropertiesAccess Of PerfusatesFly LarvaeArtificial HemolymphRhythmic Body ContractionsDissectionPosterior SegmentsBlunt Insect PinEverted Larval Body WallUltra-fine Dissection ScissorsVisceral Side Of Body Wall MusclesGlial Structures At NMJMembrane Targeted GFPGlial Specific PromotersPost-synaptic MembraneSSR (Subsynaptic Reticula)Muscle Expressed Synaptically Targeted DsRedAcutely Label Motor Neuron TerminalsPrimary Antibodies To HRPDye Diffusion PropertiesPerisynaptic Space
