Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Chapitres
- 00:00Titre
- 01:11Introduction
- 01:36Preparing for the dissection
- 04:37The dissection
- 09:15Patch-clamping muscle cells
- 11:11Modes of Muscle Stimulation
- 13:10Representative Results
- 14:41Conclusion
Application of electrophysiology to accessible synapses provides a quantifiable measure of synaptic activity, useful in analyzing synaptic mutants. This article describes a dissection method used to expose the neuromuscular junctions (NMJ) of Caenorhabditis elegans (C. elegans) and briefly discusses some of the uses to which this preparation can be applied.
Tags
Neuromuscular JunctionNeurotransmissionNeuronsChemical SignalsPost-synaptic TargetsPre-synaptic ProteinsPost-synaptic ProteinsSynaptic ConnectivityElectrical SignalsSecretory VesiclesCalcium SensingVesicle FusionPostsynaptic ReceptorsRecycling MechanismsElectrophysiological RecordingsSynaptic TransmissionMolecular ToolsGenetic ToolsC. Elegans NMJsMotor NeuronsBody Wall MusclesLocomotionUnc Mutants
