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Live Imaging of the Zebrafish Embryonic Brain by Confocal Microscopy
Journal JoVE
Biologie
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Journal JoVE Biologie
Live Imaging of the Zebrafish Embryonic Brain by Confocal Microscopy
DOI:

07:11 min

April 01, 2009

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Chapitres

  • 00:00Titre
  • 00:37Introduction
  • 01:09Preparing for Injection
  • 02:14Injecting mRNA
  • 04:12Mounting and Imaging Embryos
  • 06:14Representative Results: Confocal Imaging
  • 06:35Conclusion

Summary

Traduction automatique

In this video, we demonstrate a method by which to analyze the developing vertebrate brain in live zebrafish embryos at single cell resolution by confocal microscopy. This includes the method by which we inject the single-cell zebrafish embryo and subsequently mount and image the developing brain.

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