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Measuring Plant Cell Wall Extension (Creep) Induced by Acidic pH and by Alpha-Expansin
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Journal JoVE Biologie
Measuring Plant Cell Wall Extension (Creep) Induced by Acidic pH and by Alpha-Expansin

Measuring Plant Cell Wall Extension (Creep) Induced by Acidic pH and by Alpha-Expansin

DOI:
10.3791/1263-v

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09:51 min

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March 11, 2009

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,
1Department of Biology,Penn State University

Chapitres

  • 00:00Titre
  • 00:57Introduction
  • 01:18Growing and storing suitable plant material
  • 02:26Preparing cell wall samples
  • 04:36The extensometer setup
  • 07:08Measuring extension response to pH
  • 08:36Measuring extension response to expansin
  • 09:23Conclusion

Summary

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We demonstrate the use of a constant-force extensometer to measure long-term extension (creep) of plant cell wall specimens induced by acidic buffers and expansin protein.

Tags

MeasuringPlant Cell Wall ExtensionCreepAcidic PHAlpha-expansinAcid GrowthExtensibleLow PHPlant Hormone AuxinCell ElongationAcid-growth MechanismH+ PumpPlasma MembraneWall AcidificationExpansinsCell Wall-loosening ProteinsCell Turgor PressureEnlargementAcid-induced ExtensionCell Wall PolysaccharidesPectinsExpansin Activity AssayWall Materials Preparation TechniquesCucumber Hypocotyls

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