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Standardized Preparation of Single-Cell Suspensions from Mouse Lung Tissue using the gentleMACS Dissociator
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Biologie
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Journal JoVE Biologie
Standardized Preparation of Single-Cell Suspensions from Mouse Lung Tissue using the gentleMACS Dissociator

Standardized Preparation of Single-Cell Suspensions from Mouse Lung Tissue using the gentleMACS Dissociator

DOI:
10.3791/1266-v

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07:03 min

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July 02, 2009

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, , , ,
1Miltenyi Biotec,GmbH

Chapitres

  • 00:04Titre
  • 00:31Introduction
  • 01:01Preparing Enzyme Solutions and Buffers
  • 01:50Dissociating the Lung Tissue
  • 04:10Filtration
  • 05:35Representative Results
  • 06:07Conclusion

Summary

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Traduction automatique

Dissociating cells from specific tissue types requires specific parameters for tissue agitation to obtain a high volume of viable, culturable cells. The Miltenyi gentleMACS dissociator optimizes this task with a simple, practical protocol. In this publication the use of this apparatus on lung tissue is explained.

Tags

StandardizedPreparationSingle-cell SuspensionsGentleMACS DissociatorExperimentsCellular ResearchDissociatingWhole OrgansViable CellsReproducible MannerInstrumentPre-programmed SettingsOptimizedEfficient DissociationMouse LungsPublication

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