Journal
/
Biologie
/
Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)
Journal JoVE
Biologie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biologie
Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)

Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)

DOI:
10.3791/1325-v

•

19:16 min

•

August 05, 2009

•

,
1Department of Cell Biology,Scripps Institute

Chapitres

  • 00:05Titre
  • 00:58Introduction
  • 01:24Preparing Fluorescently Labeled Actin and Cells for Microinjection
  • 05:46Microinjection of Labeled Actin
  • 10:14Assembling the Imaging Chamber
  • 15:20Imaging Cells
  • 18:17Representative Results: Microinjected Cells
  • 18:44Conclusion

Summary

Traduction automatique

Traduction automatique

Selection, microinjection, and imaging of fluorescently-labeled F-actin via fluorescent speckle microscopy (FSM).

Tags

Fluorescent Speckle MicroscopyF-actin DynamicsLive Cell ImagingFSMActin DynamicsPtK1 CellsTurnover KineticsAssembly/disassemblyComputer Vision SoftwareQFSMFluorescent Actin ProbesMacromolecular AssembliesMicrotubulesIntermediate FilamentsAdhesion Complexes

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

Actin Co-Sedimentation Assay; for the Analysis of Protein Binding to F-Actin

Assembly, Loading, and Alignment of an Analytical Ultracentrifuge Sample Cell

Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging

Fluorescens-baserede Måling af Store betjente Calcium indtastning i levende celler: fra kulturperler kræftcellen til at Skeletmuskel Fiber

Intravital mikroskopi af Imaging Subcellulære Strukturer Live mus, der udtrykker fluorescerende proteiner

Real-time Imaging af Plant Cell Surface Dynamics med variabel vinkel epifluorescens Microscopy

Tre-dimensionel Super Resolution Mikroskopi af F-actinfilamenter af Interferometrisk fotoaktiverede Lokalisering Microscopy (iPALM)

At udnytte Live Imaging til Track kerner under Myoblast differentiering og Fusion

Undersøgelse af mitotisk og meiotisk fission gær nuklear dynamik ved fluorescens levende celle mikroskopi

Live Cell Imaging til at vurdere dynamikken i metafase timing og celle skæbne efter mitotiske spindel forstyrrelser

Read Article