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Chapitres
- 00:05Titre
- 00:08Introduction
- 00:47Cell Lysis and Digestion with Proteinase K
- 02:38DNA Extraction
- 03:56DNA Concentration and Washing
- 06:22Determination of DNA Quality and Concentration
- 07:50Cesium Chloride Gradient Centrifugation
- 14:13Representative Gel to Check DNA Quality and Concentration
- 14:40Conclusion
Tags
DNA ExtractionHigh Molecular Weight Genomic DNAPlanktonic BiomassSterivex FiltersStorage/lysis Buffer-80°C StorageCesium Chloride Density Gradient CentrifugationIncubation StepsLiberate DNARemove RNAPhenol:Chloroform ExtractionChloroform ExtractionCentrifugationProtein RemovalCell Membrane RemovalAqueous DNA ExtractBuffer ExchangePurificationCesium Chloride Density GradientSample LimitationProtocol TimeCentrifugation EquipmentHybridization Ovens
