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Generating Chimeric Zebrafish Embryos by Transplantation

Journal JoVE
Biologie

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Journal JoVE Biologie

Generating Chimeric Zebrafish Embryos by Transplantation

Generating Chimeric Zebrafish Embryos by Transplantation

DOI:

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21:01 min

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July 17, 2009

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Hilary A. Kemp, Amanda Carmany-Rampey, Cecilia Moens

¹HHMI and Division of Basic Sciences,Fred Hutchinson Cancer Research Center – FHCRC

Chapitres

00:05Titre
00:13Introduction
01:12Injecting Zebrafish Embryos at the 1-cell Stage
06:15Preparing the Transplantation Rig and Needle
08:31Transplanting Cells between Bastula Stage Embryos
13:00Transplanting Primordial Germ Cells at the Blastula Stage
15:02Transplating Cells Between Gastrula Stage Embryos
18:46Representative Results/ Outcome
20:03Conclusion

Summary

Traduction automatique

A step-by-step guide to generating targeted chimeric zebrafish embryos by transplantation at the blastula or gastrula stage.

Tags

Chimeric Embryos Zebrafish Transplantation Chimera Analysis Mosaic Chimeras Donor Embryo Host Embryo Lineage Marker Fluorescent Dye Transplantation Site Fate Maps

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