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Semi-automated Optical Heartbeat Analysis of Small Hearts
 

Semi-automated Optical Heartbeat Analysis of Small Hearts

Article DOI: 10.3791/1435-v • 12:10 min • September 16th, 2009
September 16th, 2009 •
1, 2, 1,3, 3, 1
1Development and Aging Program, The Sanford Burnham Institute for Medical Research, 2Cardiac Electrophysiology Group, Dept. of Physiology, Anatomy and Genetics, The Sanford Burnham Institute for Medical Research, 3Biology Department and Heart Institute, San Diego State University

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We have developed a Semi-automated Optical Heartbeat Analysis method (SOHA) for analyzing high speed optical recordings from Drosophila, zebrafish and embryonic mouse hearts. We demonstrate the application of our methodology to the analysis of heart function in fruit fly and embryonic mouse hearts.

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Semi-automated Optical Heartbeat Analysis Small Hearts Drosophila Zebrafish Embryonic Mouse Hearts SOHA Movement Detection Algorithm Cardiac Movements Contractile Events Relaxation Events Physiologically Relevant Readouts Systolic Intervals Diastolic Intervals Heart Rate Heartbeat Arrhythmicity Heart Diameter Measurements Diastole Systole Fractional Shortening Fractional Area Changes Digital File Output Spreadsheet Compatibility Statistical Power User Input Required Heart Function Analysis
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