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Journal JoVE Biologie
Lentivirus Production

Lentivirus Production

DOI:
10.3791/1499-v

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11:42 min

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October 02, 2009

•

,
1Diabetes Center,University of California, San Francisco – UCSF

Chapitres

  • 00:00Titre
  • 00:46Introduction
  • 01:15Transfection of 293 Cells To Produce Lentivirus
  • 03:24Lentivirus Harvest
  • 04:39Lentivirus Concentration via Ultracentrifugation
  • 07:24Lentivirus Titration Using Flow Cytometry
  • 11:17Conclusion

Summary

Traduction automatique

Traduction automatique

To make lentiviruses, DNA vectors are transfected into human 293 cells. After harvest and concentrating the supernatant, virus titer is determined by fluorescence expression with a flow cytometer.

Tags

Lentivirus ProductionRNA InterferenceGene SilencingShort Interfering RNAs (siRNA)Post-transcriptional StateShort Hairpin RNAsLentivirusesPackaging LentivirusesLentiviral Vector Production SystemsHelper Plasmid ConstructsReplication-capable VirusTransient TransfectionHuman 293 CellsIncubation PeriodVirus TiterReporter ExpressionFlow Cytometer

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