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Culture of Mouse Neural Stem Cell Precursors
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Journal JoVE Biologie
Culture of Mouse Neural Stem Cell Precursors

Culture of Mouse Neural Stem Cell Precursors

DOI:
10.3791/152-v

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27:44 min

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February 25, 2007

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, , ,
1Department of Developmental and Cell Biology,University of California, Irvine (UCI), 2Department of Pathology,University of California, Irvine (UCI), 3Department of Physiology and Biophysics,University of California, Irvine (UCI)

Chapitres

  • 00:36Preparing plate
  • 05:10Instruments
  • 05:39Dissection of E12.5 mouse
  • 07:55Removal of embryos
  • 13:20Isolation of cortex
  • 18:56Obtaining cell suspension
  • 23:21Plating neural precursors

Summary

Traduction automatique

Traduction automatique

This video describes the method used for isolation of neuroprecursors from the developing cortex of embryonic mice. The procedure for removing embryos from the uterus, dissecting the cortical tissue, and digesting the isolated cerebral cortex is shown.

Tags

Mouse Neural Stem Cell PrecursorsPrimary Neural Stem Cell CulturesCentral Nervous System DevelopmentStem Cell ResearchNervous SystemBrain DiseasesBrain InjuriesNeural DifferentiationTransdifferentiationGenetic SignalsEnvironmental SignalsStem Cell Media

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