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Chapitres
- 00:00Titre
- 01:16Introduction
- 01:49Plasmid Preparation for Electroporation
- 02:46Animal Preparation for Electroporation
- 06:12Electroporation and Transfection
- 08:31Assaying In-Vivo Transfection by Electroporation
- 08:36Transfection Efficiency
- 09:47Subcellular Protein Localization
- 12:31Expression and Targeting of the a-sub-unit of the Dihydropyridine Receptor (DHPR-a1s)
- 13:56Functional Evaluation of the Expression of EYFP-CIC1
- 15:30Conclusion
Tags
DNA TransfectionMammalian Skeletal MusclesIn Vivo ElectroporationCell BiologyTransgenic ProteinsFluorescently Tagged ConstructsMutant VariantsIn Vivo TransfectionPlasmidsLiverSkeletal MuscleBrainFlexor Digitorum BrevisInterosseus MusclesAdult MiceExperimental ParametersSoluble ProteinsStructural ProteinsMembrane ProteinsEGFPECFP3CalpainFKBP12β2a-DHPRMinidystrophinα-actininα1s-DHPRRyR1Cardiac Na/Ca2+ ExchangerNaV1.4 Na ChannelSERCA1
