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Isolation and Animal Serum Free Expansion of Human Umbilical Cord Derived Mesenchymal Stromal Cells (MSCs) and Endothelial Colony Forming Progenitor Cells (ECFCs)
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Journal JoVE Biologie
Isolation and Animal Serum Free Expansion of Human Umbilical Cord Derived Mesenchymal Stromal Cells (MSCs) and Endothelial Colony Forming Progenitor Cells (ECFCs)

Isolation and Animal Serum Free Expansion of Human Umbilical Cord Derived Mesenchymal Stromal Cells (MSCs) and Endothelial Colony Forming Progenitor Cells (ECFCs)

DOI:
10.3791/1525-v

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16:04 min

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October 08, 2009

•

,
1Stem Cell Research Unit,Medical University of Graz, Austria

Chapitres

  • 00:00Titre
  • 01:33Introduction
  • 02:14Preparation of the Umbilical Cord
  • 03:39Isolation of Endothelial Colony-Forming Progenitor Cells
  • 06:56Cell Expansion of ECFCs
  • 08:42Cell Expansion of MSCs
  • 10:11Colony Growth, Fixation, Staining and Hierarchy Analysis of ECFCs
  • 13:33Representative Cord Cells
  • 15:33Conclusion

Summary

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This protocol describes the isolation and subsequent expansion of mesenchymal stromal cells and endothelial colony forming cells without the use of animal serum to generate autologous pairs for experimental transplantation purposes.

Tags

IsolationAnimal Serum-freeHuman Umbilical CordMesenchymal Stromal CellsMSCsEndothelial Colony Forming Progenitor CellsECFCsProgenitor CellsRegenerative ProcessesTumor FormationComparative StudySame DonorRegenerative PurposesCellular TherapeuticsBench To BedsideMethod DevelopmentPooled Human Platelet LysatePHPLFetal Bovine Serum Replacement

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