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Monitoring Heart Functie in larvale Drosophila melanogaster Voor Fysiologische Studies
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Biologie
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Journal JoVE Biologie
Monitoring Heart Function in Larval Drosophila melanogaster for Physiological Studies
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Monitoring Heart Functie in larvale Drosophila melanogaster Voor Fysiologische Studies

DOI:
10.3791/1596-v

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09:37 min

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November 16, 2009

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, , , ,
1Department of Biology,University of Kentucky, Lexington

Chapitres

  • 00:00Titre
  • 00:29Introduction
  • 00:55Unrestrained Intact Larvae: The “Ant Farm” Method
  • 02:01Restrained Intact Larvae: The “Glue Method”
  • 03:52Larvae Dissection
  • 05:43Electrical Pacing
  • 07:21Field Potentials and Intracellular Recordings
  • 08:58Conclusion

Summary

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We present various ways to monitor heart function in the larva of Drosophila for assessing questions dealing with the function of gap junctions, ion channel mutations, modulation of pacemaker activity and pharmacological studies.

Tags

MonitoringHeart FunctionLarval Drosophila MelanogasterPhysiological StudiesCardiac FunctionHeart RateUnrestrained LarvaeRestrained LarvaeEnvironment ControlDissected LarvaeInternal Organs RemovalBathe Heart In CompoundsExposed HeartMembrane PotentialsIonic CurrentsMyocytesElectrical ConductionHeart TubeAdvantagesDisadvantagesFuture ExperimentsLarval Heart PreparationDrosophila Skeletal NMJIntracellular Calcium RegulationCellular FunctionAction PotentialsMyocytesSpeciesIonic DysfunctionsGenes Responsible For Diseases In Mammals

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