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Osmotic Avoidance in Caenorhabditis elegans: Synaptic Function of Two Genes, Orthologues of Human NRXN1 and NLGN1, as Candidates for Autism
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Biologie
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Journal JoVE Biologie
Osmotic Avoidance in Caenorhabditis elegans: Synaptic Function of Two Genes, Orthologues of Human NRXN1 and NLGN1, as Candidates for Autism

Osmotic Avoidance in Caenorhabditis elegans: Synaptic Function of Two Genes, Orthologues of Human NRXN1 and NLGN1, as Candidates for Autism

DOI:
10.3791/1616-v

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11:20 min

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December 11, 2009

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, ,
1Departamento de Genética,Facultad de Ciencias, Universidad de Córdoba, 2Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC)

Chapitres

  • 00:00Titre
  • 00:31Introduction
  • 01:41Fructose Ring Assay
  • 04:24Behavioral Analysis of Knockout Animals
  • 05:33RNAi Feeding
  • 07:23Worm Handling and Scoring
  • 08:24Behavior Analysis of Knockdown Animals
  • 09:30Summary

Summary

Traduction automatique

Traduction automatique

Neurexins and neuroligins are membrane-neuron adhesion proteins which perform essential roles in synaptic differentiation and transmission. Neuroligin deficient mutants of C. elegans are defective in detecting osmotic strength, but when they also contain a mutation in the gene coding neurexin, they recover the wild type phenotype.

Tags

Osmotic AvoidanceCaenorhabditis ElegansSynaptic FunctionGenesNRXN1NLGN1AutismNeurexinsNeuroliginsExcitatory SynapsesInhibitory SynapsesPresynaptic MembranesPostsynaptic MembranesNeuronal ConnectionsDevelopment Of Human Nervous SystemAutism Spectrum DisordersExperimental ToolLaboratory ExperimentationNrx-1 GeneNlg-1 GeneAlpha-neurexin-1 ProteinNeuroligin-1 ProteinFunctional DomainsAmphid Sensory Organ

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