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1Department of Otology and Laryngology,Harvard Medical School, 2Eaton-Peabody Laboratory,Massachusetts Eye and Ear Infirmary, 3Department of Communication Sciences and Disorders,Emerson College, 4Program in Speech and Hearing Bioscience and Technology, Division of Health Science and Technology,Harvard
Chapitres
- 00:00Titre
- 00:05Introduction
- 00:44Procedure Overview
- 01:45Day 1: Coating of Glass Coverslips and Heat Sterilization Tools
- 02:41Day 2: Isolation and Culture of the Murine Organ of Corti
- 08:15Applications for Isolated Organ of Corti
- 11:08Day 4: Visualization of Electroporated Cells
- 11:37Conclusion
इस प्रक्रिया के साथ या बिना सर्पिल किनारी और सर्पिल नाड़ीग्रन्थि न्यूरॉन्स Corti के murine अंग के अलगाव और संस्कृति के लिए एक विधि का वर्णन करता है. हम भी electroporation द्वारा Corti explant के अंग में एक exogenous रिपोर्टर जीन की अभिव्यक्ति के लिए एक विधि का प्रदर्शन.
Tags
Primary CulturePlasmid ElectroporationMurine Organ Of CortiSensory EpitheliumAuditionCochleaInner EarHair CellsMechanosensory CellsInner Hair CellsOuter Hair CellsBasilar MembraneSensorineural Hearing LossAuditory ResearchOrgan ExplantGene ExpressionStereocilia MotilityCell And Molecular BiologyHair Cell RegenerationIsolation And Culture MethodNeonatal MiceTemporal Bone Isolation
